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When to take a pregnancy test, and what hCG levels mean

Quick answer

Take a home pregnancy test from the first day of your missed period, ideally with the first pee of the morning. Tests detect hCG, a hormone your body makes once a fertilised egg implants. Testing earlier can give a false negative. A faint test line usually means you're pregnant. If the test is negative but your period still hasn't come, test again in 3 days.

Key facts

Best time to testFirst day of a missed period or later
hCG startsAfter implantation, 6 to 12 days after ovulation
Early hCGRoughly doubles every 2 to 3 days
Faint lineUsually positive
Common mistakeTesting too early

How pregnancy tests work

Once a fertilised egg implants in the womb, the developing placenta makes human chorionic gonadotropin (hCG). It shows up in blood first, then in urine. Home tests contain antibodies that change colour when hCG is present.

9 days after ovulationOften negative12 days after ovulationFaint lineDay of missed periodClear lineTC
How the test line appears. C is the control line and must always show. T is the test line: even a faint one usually means you're pregnant. hCG roughly doubles every 2 to 3 days early on, so the line darkens.

When to take the test

  • Best: on or after the first day of your missed period. Most tests are very accurate from this point.
  • "Early" tests can pick up hCG a few days before your period, but a negative this early doesn't rule out pregnancy.
  • Irregular cycles: test 21 days after unprotected sex.
  • Time of day: morning urine is most concentrated, which matters most in the first days.

Reading your result

  • Two lines, even faint: you're very likely pregnant. Read it within the time on the packet.
  • One line (control only): negative. Test again in 3 days if your period doesn't come.
  • No control line: the test didn't work. Use another.
  • Evaporation line: a colourless line that appears after the reading time. Ignore results read late.
  • Digital tests show words, which removes guesswork but can be slightly less sensitive.

hCG levels week by week

Doctors measure hCG in a blood test in milli-international units per millilitre (mIU/mL). The normal range is very wide, so one number tells you little. In early pregnancy, doctors look at whether it roughly doubles over 48 to 72 hours.

Weeks since last periodTypical hCG range (mIU/mL)
3 weeks5 – 50
4 weeks5 – 426
5 weeks18 – 7,340
6 weeks1,080 – 56,500
7 – 8 weeks7,650 – 229,000
9 – 12 weeks25,700 – 288,000
13 – 16 weeks13,300 – 254,000
17 – 24 weeks4,060 – 165,400

Under 5 mIU/mL is generally negative; over 25 is generally positive. hCG peaks around 8 to 11 weeks, then falls and levels off.

Why a test can be wrong

False negatives are common and happen when you test too early, drink a lot before testing, or read the result too soon. False positives are rare. They can follow a very early miscarriage (chemical pregnancy), fertility injections containing hCG, or rare medical conditions. Antibiotics and the contraceptive pill don't affect results.

After a positive test

Congratulations. Work out your due date with the calculator, start folic acid, and book your first appointment. Read early signs of pregnancy and the first trimester guide for what comes next.

Frequently asked questions

Can I take a pregnancy test at night?
Yes, but your urine is more diluted. If it's negative and your period is late, retest with morning urine.
Does a darker line mean twins?
Not reliably. Twins can raise hCG, but line darkness varies with urine concentration and the brand.
How soon will a blood test show pregnancy?
A blood test can detect hCG a few days earlier than urine, around 10 days after conception.

Sources

  • NHS: Doing a pregnancy test
  • American Pregnancy Association: hCG levels
  • ACOG: Pregnancy tests

General information, not medical advice. Medical disclaimer · How we review content

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